Thursday, May 28, 2026

GLUCAGON- exam ready notes

 

GLUCAGON

Introduction

Glucagon is a peptide hormone secreted by the α-cells (alpha cells) of the Islets of Langerhans of the pancreas. It is the chief hyperglycemic hormone of the body and acts antagonistically to insulin. Its main function is to increase blood glucose level during fasting and hypoglycemia. Glucagon:

  • Mobilizes stored glucose
  • Maintains blood glucose during fasting
  • Stimulates glycogen breakdown
  • Stimulates glucose formation in liver

Pancreas as an Endocrine Gland

The pancreas is both:

  • Exocrine gland → secretes digestive enzymes
  • Endocrine gland → secretes hormones

The endocrine part contains Islets of Langerhans.

Cells of Islets of Langerhans

Cell Type

Hormone Secreted

Main Function

α-cells

Glucagon

Raises blood glucose

β-cells

Insulin

Lowers blood glucose

δ-cells

Somatostatin

Inhibitory hormone

PP cells

Pancreatic polypeptide

GI regulation

Chemical Nature of Glucagon

  • Glucagon is a peptide hormone
  • It consists of 29 amino acids
  • It is derived from a precursor called: Proglucagon

Synthesis of Glucagon

Steps

Step-1. Formation of Proglucagon

  • Synthesized in α-cells

Step-2. Processing by Enzymes

  • Processed by prohormone convertase-2 (PC2)

Step-3. Formation of Glucagon

  • Mature glucagon hormone released

Stimuli For Glucagon Secretion

Main Stimulus

Low Blood Glucose (Hypoglycemia)

Glucagon secretion increases during:

  • Fasting
  • Starvation
  • Exercise
  • Protein-rich meals

Factors Affecting Glucagon Secretion

Factors Increasing Secretion

  • Hypoglycemia
  • Amino acids
  • Exercise
  • Stress
  • Sympathetic stimulation

Factors Decreasing Secretion

  • Hyperglycemia
  • Insulin
  • Somatostatin
  • GLP-1

Mechanism of Glucagon Secretion

When blood glucose falls:

↓ Blood glucose

α-cells stimulated

Glucagon secretion

Liver stimulated

Glucose released into blood

Mechanism of Action of Glucagon

Glucagon acts through a: G-protein coupled receptor (GPCR)

The receptor is mainly present in:

  • Liver
  • Adipose tissue
  • Heart
  • Kidney

Second Messenger System

Glucagon works through: cAMP pathway

Steps

  1. Glucagon binds receptor
  2. Adenyl cyclase activated
  3. cAMP formed
  4. Protein kinase activated
  5. Metabolic enzymes activated

Target Organs of Glucagon

Main target organ: Liver

Other targets: Adipose tissue, Heart

Functions of Glucagon

1. Effects on Carbohydrate Metabolism

Glucagon raises blood glucose level.

Major Actions

A. Glycogenolysis

Breakdown of glycogen into glucose.

B. Gluconeogenesis

Formation of glucose from non-carbohydrate sources.

C. Inhibits Glycogenesis

Prevents storage of glucose as glycogen.

D. Inhibits Glycolysis

Decreases glucose breakdown in liver.

Overall Effect

↑ Blood glucose level

Therefore, glucagon is called: “Hyperglycemic Hormone”

2. Effects on Fat Metabolism

Glucagon promotes fat breakdown.

Actions

  • Stimulates lipolysis
  • Increases fatty acid oxidation
  • Promotes ketogenesis

3. Effects on Protein Metabolism

  • Promotes amino acid utilization
  • Supports gluconeogenesis from amino acids

4. Effects During Fasting

During fasting:

  • Blood glucose falls
  • Glucagon secretion rises
  • Liver releases glucose

This maintains: Glucose homeostasis

Glucose Homeostasis

Blood glucose is controlled mainly by:

  • Insulin
  • Glucagon

After Meals

  • Insulin predominates
  • Glucose stored

During Fasting

  • Glucagon predominates
  • Glucose mobilized

Insulin vs Glucagon

Feature

Insulin

Glucagon

Secreted by

β-cells

α-cells

Blood glucose

Decreases

Increases

Main action

Glycogenesis

Glycogenolysis

Nature

Hypoglycemic

Hyperglycemic

Fasting state

Decreases

Increases

Glucagon in Starvation

During prolonged starvation:

  • Glycogen stores depleted
  • Glucagon stimulates gluconeogenesis
  • Fat metabolism increases
  • Ketone bodies formed

Clinical Importance of Glucagon

1. Hypoglycemia

Glucagon is used therapeutically to treat: Severe hypoglycemia

Especially in: Diabetic patients receiving insulin

2. Diabetes Mellitus

In diabetes:

  • Glucagon secretion may become excessive
  • Hyperglycemia worsens

3. Glucagonoma

Definition

Rare tumor of α-cells producing excess glucagon.

Features

  • Hyperglycemia
  • Weight loss
  • Skin rash
  • Diabetes mellitus

Glucagon and Exercise

During exercise:

  • Muscles consume glucose
  • Blood glucose may fall
  • Glucagon secretion increases
  • Liver releases glucose

Important Metabolic Effects

Process

Effect of Glucagon

Glycogenolysis

Increased

Gluconeogenesis

Increased

Glycogenesis

Decreased

Glycolysis

Decreased

Lipolysis

Increased

Ketogenesis

Increased

 

High-Yield Flowchart

Low Blood Glucose

α-cells stimulated

Glucagon secretion

Liver glycogen breakdown

Glucose released

Increase in Blood Glucose

Important Facts

  • Glucagon is secreted by α-cells
  • It is a peptide hormone
  • Contains 29 amino acids
  • Raises blood glucose
  • Acts mainly on liver
  • Works via cAMP pathway
  • Opposes insulin action
  • Stimulates glycogenolysis and gluconeogenesis

Point to Remember

“Glucagon is a peptide hormone, and plays an important role in maintaining normal blood glucose levels.”

MEMORY TRICK

“GLUCAGON = GLUCOSE GONE UP”

Glucagon → Glucose ↑

Insulin → Glucose ↓

 

Quick Revision Table

Feature

Glucagon

Secreted by

α-cells

Nature

Peptide hormone

Amino acids

29

Main target

Liver

Main action

Raises blood glucose

Mechanism

cAMP pathway

Opposes

Insulin

One-Line Summary

“Glucagon is a hyperglycemic peptide hormone secreted by pancreatic α-cells that increases blood glucose mainly by stimulating glycogenolysis and gluconeogenesis in the liver.”

 


Wednesday, May 27, 2026

INSULIN= short notes

 


INSULIN

Introduction

Insulin is a peptide hormone secreted by the β-cells of the Islets of Langerhans of the pancreas. It is the chief hypoglycemic hormone of the body and plays a central role in the regulation of glucose homeostasis.

It promotes:

  • Uptake of glucose by cells
  • Storage of glucose as glycogen
  • Synthesis of fats and proteins
  • Growth and anabolic activities

Pancreas as an Endocrine Gland

The pancreas is a mixed gland:

  • Exocrine part → secretes digestive enzymes
  • Endocrine part → Islets of Langerhans

There are about 1–2 million Islets of Langerhans in humans, forming approximately 1–2% of pancreatic tissue.

Cells of Islets of Langerhans

Cell Type

Hormone Secreted

Function

α-cells

Glucagon

Raises blood glucose

β-cells

Insulin

Lowers blood glucose

δ-cells

Somatostatin

Inhibitory hormone

PP cells

Pancreatic polypeptide

GI regulation

 

Chemical Nature of Insulin

  • Insulin is a protein/peptide hormone.
  • It contains 51 amino acids
  • Consists of:
    • A-chain → 21 amino acids
    • B-chain → 30 amino acids
  • Chains are connected by disulfide bonds

Structure

A-chain (21 aa) ← disulfide bonds → B-chain (30 aa)

Synthesis of Insulin

Insulin is synthesized in β-cells in several steps.

Steps of Synthesis

1. Formation of Preproinsulin

  • Initial inactive precursor
  • Synthesized on ribosomes of rough ER

2. Formation of Proinsulin

  • Signal peptide removed
  • Proinsulin contains:
    • A-chain
    • B-chain
    • Connecting peptide (C-peptide)

3. Formation of Insulin

  • C-peptide removed in Golgi apparatus
  • Mature insulin formed

Preproinsulin → Proinsulin → Insulin + C-peptide

C-peptide and insulin are released in equal amounts.

C-Peptide

Importance

  • Marker of endogenous insulin secretion
  • Helps distinguish:
    • Natural insulin production
    • Injected insulin use

Clinical Significance

  • High C-peptide → body producing insulin
  • Low C-peptide → β-cell failure

Mechanism of Insulin Secretion

Main Stimulus

Increased Blood Glucose

Mechanism

  1. Glucose enters β-cells via GLUT transporters
  2. ATP production increases
  3. ATP-sensitive K⁺ channels close
  4. Cell membrane depolarizes
  5. Ca²⁺ channels open
  6. Calcium influx occurs
  7. Insulin released by exocytosis

Flowchart

↑ Blood glucose

Glucose enters β-cell

↑ ATP production

Closure of K+ channels

Depolarization

Opening of Ca2+ channels

Insulin secretion

 

Factors Affecting Insulin Secretion

Factors Increasing Insulin Secretion

  • Increased blood glucose
  • Amino acids
  • Fatty acids
  • Glucagon
  • Gastrointestinal hormones
  • Parasympathetic stimulation

Factors Decreasing Insulin Secretion

  • Fasting
  • Sympathetic stimulation
  • Somatostatin
  • Low blood glucose

Mechanism of Action of Insulin

Insulin acts through a receptor tyrosine kinase present on target cell membranes.

Steps

  1. Insulin binds receptor
  2. Receptor activated
  3. Phosphorylation cascade initiated
  4. GLUT-4 transporters move to membrane
  5. Glucose uptake increases

Target Organs of Insulin

Main target tissues:

  • Liver
  • Skeletal muscle
  • Adipose tissue

Functions of Insulin

1. Effects On Carbohydrate Metabolism

Insulin lowers blood glucose level.

Actions

In Liver

  • Increases glycogenesis
  • Decreases glycogenolysis
  • Decreases gluconeogenesis

In Muscle

  • Increases glucose uptake
  • Increases glycogen synthesis

In Adipose Tissue

  • Increases glucose utilization

Overall Effect

↓ Blood glucose level

Insulin is therefore called a: “Hypoglycemic Hormone”

2. Effects On Fat Metabolism

Insulin promotes fat storage.

Actions

  • Increases lipogenesis
  • Decreases lipolysis
  • Promotes triglyceride storage

Result

  • Fat accumulation
  • Energy storage

3. Effects On Protein Metabolism

Insulin is anabolic.

Actions

  • Increases amino acid uptake
  • Increases protein synthesis
  • Decreases protein breakdown

4. Effects On Electrolytes

  • Increases K⁺ entry into cells
  • Promotes phosphate uptake

Overall Metabolic Effects

Metabolism

Effect of Insulin

Carbohydrate

↓ Blood glucose

Protein

↑ Protein synthesis

Fat

↑ Fat storage

Electrolytes

↑ K⁺ uptake

Glucose Homeostasis

Blood glucose is regulated jointly by:

  • Insulin
  • Glucagon

Fed State

  • Insulin dominates
  • Glucose stored

Fasting State

  • Glucagon dominates
  • Glucose released

INSULIN AND GLUCAGON: COMPARISON

Feature

Insulin

Glucagon

Secreted by

β-cells

α-cells

Nature

Peptide hormone

Peptide hormone

Blood glucose

Decreases

Increases

Action

Hypoglycemic

Hyperglycemic

Glycogenesis

Stimulates

Inhibits

Glycogenolysis

Inhibits

Stimulates

Diabetes Mellitus

Definition

A metabolic disorder characterized by:

  • Hyperglycemia
  • Glucose in urine
  • Impaired insulin secretion/action

Types of Diabetes Mellitus

Type 1 Diabetes Mellitus- Insulin Dependent Diabetes Mellitus (IDDM)

Cause

  • Autoimmune destruction of β-cells
  • Absolute insulin deficiency

Features

  • Usually in children
  • Requires insulin therapy

Type 2 Diabetes Mellitus- Non Insulin Dependent Diabetes Mellitus (NIDDM)

Cause

  • Insulin resistance
  • Relative insulin deficiency

Features

  • Common in adults
  • Associated with obesity

Symptoms of Diabetes

Classical Symptoms

3 Ps

  • Polyuria
  • Polydipsia
  • Polyphagia

Other symptoms:

  • Weight loss
  • Fatigue
  • Blurred vision

Complications of Diabetes

Acute

Chronic

  • Retinopathy
  • Nephropathy
  • Neuropathy
  • Cardiovascular disease

Insulin Therapy

Diabetic patients can be treated successfully using insulin.

Routes

  • Usually subcutaneous injection

Types of Insulin

Type

Onset

Rapid acting

Minutes

Short acting

30–60 min

Intermediate acting

Hours

Long acting

Prolonged

Hypoglycemia

Definition

Low blood glucose level due to excess insulin.

Symptoms

  • Sweating
  • Tremors
  • Hunger
  • Confusion
  • Coma (severe)

Important Points

  • Insulin is secreted by β-cells
  • Insulin is a protein hormone
  • Insulin decreases blood glucose
  • Insulin promotes glycogenesis
  • Glucagon is antagonistic to insulin
  • C-peptide released with insulin
  • Insulin deficiency causes diabetes mellitus
  • GLUT-4 transporters increase glucose uptake
  • Insulin acts via tyrosine kinase receptor

High-Yield Flowchart

High Blood Glucose

β-cells stimulated

Insulin secretion

Glucose uptake by tissues

Glycogenesis + Lipogenesis

Decrease in Blood Glucose

Quick Revision Table

Feature

Insulin

Secreted by

β-cells

Nature

Peptide hormone

Amino acids

51

Main action

Lowers blood glucose

Receptor

Tyrosine kinase

Deficiency

Diabetes mellitus

Opposite hormone

Glucagon

One-Line Summary

“Insulin is an anabolic peptide hormone secreted by pancreatic β-cells that lowers blood glucose by promoting uptake and storage of glucose.”