Monday, January 9, 2023

Liver- Structure

 Liver- Structure

Introduction

The liver is the largest organ in the body and acts as a well-equipped biochemical laboratory where practically metabolism of all the nutritional substances, viz., carbohydrates, proteins, lipids, vitamins and minerals takes place as well as heat is produced.  The liver is both a secretory and an excretory gland. The liver is an accessory digestive organ that produces bile, an alkaline fluid containing cholesterol and bile acids, which helps the breakdown of fat.

liver's highly specialized tissue, consisting of mostly hepatocytes, regulates a wide variety of high-volume biochemical reactions, including the synthesis and breakdown of small and complex molecules, many of which are necessary for normal vital functions. Estimates regarding the organ's total number of functions are estimated to being around 500.

Anatomy and histology

The liver is a reddish-brown, wedge-shaped organ with two lobes of unequal size and shape. A human liver normally weighs approximately 1.5 kg and has a width of about 15 cm. There is considerable size variation between individuals, with the standard reference range as follows- 

·        Men

970–1,860 g 

·        Women

600–1,770 g

 

It is both the heaviest internal organ and the largest gland in the human body. Located in the right upper quadrant of the abdominal cavity, it rests just below the diaphragm, to the right of the stomach and overlies the gallbladder.

It is almost a solid organ consisting of several lobes; each lobe is made up of numerous lobules. Under the microscope, each lobule is found to be composed of rows of polygonal cells from the centre-like the spokes, radiating of the lobule being delineated by the presence of portal triads.

The periphery of a wheel is occupied by the central (intra lobular) vein. The portal vein and hepatic artery enter into, and bile duct and hepatic vein emerge out of the liver through a connective tissue sheath (Glisson's capsule). After entering centre they branch repeatedly.

The liver cells are arranged in the form of plates having thickness of single-cell diameter throughout this structure which makes it a sponge like structure. The cell plates are tunneled by a communicating, system of cavities or lacunae. These contain hepatic sinusoids, endothelial cells and phagocytic cells of RE System called Kupffer cells. The narrow space separating the sinusoidal wall from the liver cell plates is known as Disse's space.

Gross Anatomy

The liver is divided into two parts when viewed from above –

·        a right lobe

·        a left lobe

And four parts when viewed from below-

·        Left

·        Right 

·        Caudate

·        Quadrate Lobes

Important Relations

Anteriorly- Diaphragm, right and left costal margins, right and left pleura and lower margins of both lungs, xiphoid process, and anterior abdominal wall in the subcostal angle

Posteriorly- Diaphragm, right kidney, hepatic flexure of the colon, duodenum, gallbladder, inferior vena cava, and esophagus and fundus of the stomach Peritoneal Ligaments of the Liver

1. Falciform Ligament- The falciform ligament, which is a two-layered fold of the peritoneum, ascends from the umbilicus to the liver. It has a sickle-shaped free margin that contains the ligamentum teres, the remains of the umbilical vein. The falciform ligament passes on to the anterior and then the superior surfaces of the liver and then splits into two layers.

 a. The right layer forms the upper layer of the coronary ligament;

b. the left layer forms the upper layer of the left triangular ligament.

The right extremity of the coronary ligament is known as the right triangular ligament.

Bare Area of the Liver- The peritoneal layers forming the coronary ligament are widely separated, leaving an area of liver devoid of peritoneum. This area is called the bare area of the liver.

2. Ligamentum Teres- The ligamentum teres passes into a fissure on the visceral surface of the liver and joins the left branch of the portal vein in the porta hepatis.

3. Ligamentum Venosum. The ligamentum venosum, a fibrous band that is the remains of the ductus venosus, is attached to the left branch of the portal vein and ascends in a fissure on the visceral surface of the liver to be attached above to the inferior vena cava.

Liver Segments

liver segment is one of eight segments of the liver as described in the widely used Couinaud classification (named after Claude Couinaud) in the anatomy of the liver. This system divides the lobes of the liver into eight segments based on a transverse plane through the bifurcation of the main portal vein, arranged in a clockwise manner starting from the caudate lobe. There are four lobes of the liver. The Couinaud classification of liver anatomy then further divides the liver into eight functionally independent segments.

Each segment has its own vascular inflow, outflow and biliary drainage. In the centre of each segment there is a branch of the portal vein, hepatic artery and bile duct. In the periphery of each segment there is vascular outflow through the hepatic veins. The division of the liver into independent units means that segments can be resected without damaging the remaining segments. 

Lobes of Liver

Left lobe

The left lobe is smaller and more flattened than the right. It is situated in the epigastric, and left hypochondriac regions of the abdomen. Its upper surface is slightly convex and is molded on to the diaphragm; its under surface presents the gastric impression and omental tuberosity.

Right lobe

The right lobe is six times the size of the left lobe. It occupies the right hypochondrium, on its posterior surface by the ligamentum venosum for the cranial (upper) half and by the ligamentum teres hepatis (Round ligament of liver) for the caudal (under) half. The ligamentum teres hepatis turns around the inferior margin of the liver to come out ventral in the falciform ligament.

The right lobe is functionally separated from the left lobe by the middle hepatic vein. From a functional perspective (one that takes the arterial, portal venous, and systemic venous anatomy into account) the falciform ligament separates the medial and lateral segments of the left hepatic lobe.

The right lobe is of a somewhat quadrilateral form. It’s under and posterior surfaces being marked by three fossæ- the fossa for the portal vein, the fossa for the gall-bladder and the fossae for the inferior vena cava. These separate the right lobe into two smaller lobes on its left posterior part: the quadrate lobe and the caudate lobe.

Quadrate lobe

The quadrate lobe is an area of the liver situated on the undersurface of the medial segment left lobe (Couinaud segment IVb), bounded in front by the anterior margin of the liver, behind by the porta hepatis, on the right by the fossa for the gall-bladder, and on the left by the fossa for the umbilical vein. It is oblong in shape, its anteroposterior diameter being greater than its transverse.

Caudate lobe

The caudate lobe (posterior hepatic segment-I) is situated upon the posterosuperior surface of the liver on the right lobe of the liver, opposite the tenth and eleventh thoracic vertebrae.

The caudate lobe of the liver is bounded below by the porta hepatis, on the right by the fossa for the inferior vena cava, and on the left by the fossa for the ductus venosus and the physiological division of the liver, called the ligamentum venosum. It looks backward, being nearly vertical in position; it is longer from above downward than from side to side, and is somewhat concave in the transverse direction. It is situated behind the porta, and separates the fossa for the gall-bladder from the commencement of the fossa for the inferior vena cava

The caudate lobe is named after the tail-shaped hepatic tissue (cauda; Latin, tail) papillary process of the liver, which arise from its left side. It also has a caudate process (that is not tail-like shaped) arising from its right side, which provides surface continuity between the caudate lobe and the visceral surface of the anatomical right lobe of the liver. The caudate process is a small elevation of the hepatic substance extending obliquely and laterally, from the lower extremity of the caudate lobe to the undersurface of the right lobe.

Caudate lobe

Segment I is the caudate lobe and is situated posterior l. It may receive its supply from both the right and the left branches of portal vein. It contains one or more hepatic veins which drain directly into the inferior vena cava. The caudate lobe is a separate structure which receives blood flow from both the right- and left-sided vascular branches.

Left lobe

Segments II and III are both medial to the falciform ligament with II superior to the portal venous supply and III inferior. Segment IV is found lateral to the falciform ligament and is subdivided into IVa (superior) and IVb (inferior).

Right lobe

Segments V to VIII make up the right part of the liver.

·         Segment V - the most medial and inferior

·         Segment VI - located more posteriorly

·         Segment VII - located above segment VI

·         Segment VIII - above segment V in the superiomedial position

Quadrate lobe

The fissure for the round ligament of the liver (ligamentum teres) separates the medial and lateral parts of segment IV. The inferior medial segment (IVb) is called the quadrate lobe.

Impressions

Several impressions on the surface of the liver accommodate the various adjacent structures and organs. Underneath the right lobe and to the right of the gallbladder fossa are two impressions, one behind the other and separated by a ridge. The one in front is a shallow colic impression, formed by the hepatic flexure and the one behind is a deeper renal impression accommodating part of the right kidney and part of the suprarenal gland.

The suprarenal impression is a small, triangular, depressed area on the liver. It is located close to the right of the fossa, between the bare area and the caudate lobe, and immediately above the renal impression. The greater part of the suprarenal impression is devoid of peritoneum and it lodges the right suprarenal gland.

Medial to the renal impression is a third and slightly marked impression, lying between it and the neck of the gall bladder. This is caused by the descending portion of the duodenum, and is known as the duodenal impression.

The inferior surface of the left lobe of the liver is present behind and to the left of the gastric impression. This is molded over the upper front surface of the stomach, and to the right of this is a rounded eminence, the tuber omentale, which fits into the concavity of the lesser curvature of the stomach and lies in front of the anterior layer of the lesser omentum.

Borders and Surfaces

It has two borders-

·        Anterior border

·        Posterior border

It has two surfaces-

·        Superior or diaphragmatic surface

·        Inferior or visceral surface

On the diaphragmatic surface, apart from a triangular bare area where it connects to the diaphragm, the liver is covered by a thin, double-layered membrane, the peritoneum that helps to reduce friction against other organs. This surface covers the convex shape of the two lobes where it accommodates the shape of the diaphragm. The peritoneum folds back on itself to form the falciform ligament and the right and left triangular ligaments.

The visceral surface is uneven and concave. It is covered in peritoneum apart from where it attaches the gallbladder and the porta hepatis. The fossa of gall bladder lies to the right of the quadrate lobe, occupied by the gallbladder with its cystic duct close to the right end of porta hepatis.

Functional Anatomy

The central area or hepatic hilum, includes the opening known as the porta hepatis which carries the common bile duct and common hepatic artery, and the opening for the portal vein. The duct, vein, and artery divide into left and right branches, and the areas of the liver supplied by these branches constitute the functional left and right lobes.

The functional lobes are separated by the imaginary plane, Cantlie's line, joining the gallbladder fossa to the inferior vena cava. The plane separates the liver into the true right and left lobes. The middle hepatic vein also demarcates the true right and left lobes.

The right lobe is further divided into an anterior and posterior  segment by the right hepatic vein.

The left lobe is divided into the medial  and lateral segments by the left hepatic vein.

The hilum of the liver is described in terms of three plates that contain the bile ducts and blood vessels. The contents of the whole plate system are surrounded by a sheath. 

The three plates are-

·        Hilar Plate

·        Cystic Plate 

·        Umbilical Plate 

The plate system shows many anatomical variations to be found in the liver.

Blood Supply

Arteries

The hepatic artery, a branch of the celiac artery, divides into right and left terminal branches that enter the porta hepatis.

Veins

The portal vein divides into right and left terminal branches that enter the porta hepatis behind the arteries. The hepatic veins (three or more) come out from the posterior surface of the liver and drain into the inferior vena cava.

Blood Circulation through the Liver

The blood vessels conveying blood to the liver are the hepatic artery (30%) and portal vein (70%). The hepatic artery brings oxygenated blood to the liver, and the portal vein brings venous blood rich in the products of digestion, which have been absorbed from the gastrointestinal tract. The arterial and venous blood is conducted to the central vein of each liver lobule by the liver sinusoids. The central veins drain into the right and left hepatic veins, and these leave the posterior surface of the liver and open directly into the inferior vena cava.

Lymph Drainage

The liver produces a large amount of lymph—about one third to one half of all body lymph. The lymph vessels leave the liver and enter several lymph nodes in the porta hepatis. The efferent vessels pass to the celiac nodes. A few vessels pass from the bare area of the liver through the diaphragm to the posterior mediastinal lymph nodes.

Nerve Supply

Sympathetic and parasympathetic nerves form the celiac plexus. The anterior vagal trunk gives rise to a large hepatic branch, which passes directly to the liver.

Histology of Liver

Microscopically, each liver lobe is seen to be made up of hepatic lobules. Lobules are the functional units of the liver. Each lobule is made up of millions of hepatic cells (hepatocytes), which are the basic metabolic cells. The lobules are held together by a fine, dense, irregular, fibroelastic connective tissue layer extending from the fibrous capsule covering the entire liver known as Glisson's capsule.

This extends into the structure of the liver by accompanying the blood vessels, ducts, and nerves at the hepatic hilum. The whole surface of the liver, except for the bare area, is covered in a serous coat derived from the peritoneum, and this firmly adheres to the inner Glisson's capsule.

The lobules are roughly hexagonal, and consist of plates of hepatocytes, and sinusoids radiating from a central vein towards an imaginary perimeter of interlobular portal triads. The central vein joins to the hepatic vein to carry blood out from the liver.

A distinctive component of a lobule is the portal triad, which can be found running along each of the lobule's corners. The portal triad consists of the hepatic artery, the portal vein, and the common bile duct.

Histology shows two major types of liver cells- 

·        Parenchymal cells 

·        Non parenchymal cells

About 70–85% of the liver volume is occupied by parenchymal hepatocytes. Non parenchymal cells constitute 40% of the total number of liver cells but only 6.5% of its volume. The liver sinusoids are lined with two types of cell, sinusoidal endothelial cells, and phagocytic Kupffer cellsHepatic stellate cells are non parenchymal cells found in the perisinusoidal space, between a sinusoid and a hepatocyte. Additionally, intrahepatic lymphocytes are also found.

Under the microscope, each lobule is found to be composed of rows of polygonal cells from the centre-like the spokes, radiating of the lobule being delineated by the presence of portal triads. The periphery of a wheel is occupied by the central (intra lobular) vein. The portal vein and hepatic artery enter into, and bile duct and hepatic vein emerge out of the liver through a connective tissue sheath (Glisson's capsule). After entering centre they branch repeatedly.

The liver cells are arranged in the form of plates having thickness of single-cell diameter throughout this structure which makes it a sponge like structure. The cell plates are tunneled by a communicating, system of cavities or lacunae. These contain hepatic sinusoids, endothelial cells and phagocytic cells of RE System called Kupffer cells. The narrow space separating the sinusoidal wall from the liver cell plates is known as Disse's space.


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